Bisphenol-A
- The text on this page is taken from an equivalent page of the IEHIAS-project.
BPA is extensively employed in the production of epoxy resins and polycarbonate plastics and has estrogenic activity
Bisphenol-A as a biomarker
Sample collection and storage
Matrix:
BPA can be analyzed in blood (serum, cord, red blood cells), in breast milk, urine and feces
Kinetics:
- Food is the main uptake route of BPA
- Exposure from water and atmospheric pollution may be relevant
- In rats, BPA is rapidly absorbed, metabolized and excreted (2-3 days)
- Data from humans show BPA metabolization and excretion within 24 hours, with virtually no accumulation
Sampling conditions:
Both blood and breast milk sampled can be gathered and stored using standard procedures. Samples are stored at -20°C
Sample measurement
Analytical aspects:
- Standard measurement methods include combinations of GC, LC, MS and HPLC techniques;
- Detection limits in breast milk are reported below 1 ng/ml, and 0.6 ng/ml in fresh and seawater
Performance characteristics:
Recoveries of spiked BPA in cord blood are 65-120% and coefficients of variation are blow 15%. Intra- and inter-day variability is generally below 15%.
Validation:
Validated analytical methods are developed for cord blood, urine, and other matrices
Confounding factors:
BPA patterns may depend on food consumption patterns
Data interpretation
Concentrations reported in literature:
- Breast milk: 0.8-1.1 ng/ml
- Urine: 1.28 ng/ml
- Cord blood: ND – 4.05 ng/ml
- Blood levels: 0.41 ng/ml
Dose-response/effect relationships:
Significant increase in prostate size, decreased epididymal weight and longer anogenital distance in mice
Time trend, geographical variation, susceptibel groups:
No consistent data are available