Talk:Biliverdin reductase A activity
Total BVRA activity is better than specific
| Fact discussion: . |
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| Opening statement: Total activity is a better measure of actual BVRA activity than putatively specific activity.
Closing statement: Accepted. (Resolved, i.e., a closing statement has been found and updated to the main page.) |
| Argumentation:
----Background info: . BVRA is an enzyme that reduces biliverdin to bilirubin. NADPH is consumed in the reaction. Definitions: Total activity is the observed increase in absorbance (460 nm) per minute in reaction mix consisting of buffer, biliverdin, cytosol and NADPH. Blank is the observed increase in absorbance (460nm) per minute in reaction mix consisting of buffer, biliverdin and cytosol. Putatively specific activity is Total activity - blank. --Jouni 13:27, 1 September 2010 (UTC) (type: truth; paradigms: science: comment) ----Discussion history: . The original discussion from [1] was formalised. --Jouni 13:27, 1 September 2010 (UTC) (type: truth; paradigms: science: comment) ←--1: . Energy sources (NADPH) are available in the cytosol. --Jouni 13:27, 1 September 2010 (UTC) (type: truth; paradigms: science: defence) ←--2: . Total activity is a specific measure of BVRA. --Jouni 13:27, 1 September 2010 (UTC) (type: truth; paradigms: science: defence)
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